Bromophenol blue spectrophotometry. Bromothymol Blue Spectrophotometry 2022-10-15

Bromophenol blue spectrophotometry Rating: 8,8/10 562 reviews

Bromophenol blue is a compound that is commonly used as a pH indicator in spectrophotometry, a technique that measures the amount of light absorbed by a substance. Spectrophotometry is a widely used analytical tool in many fields, including chemistry, biochemistry, and environmental science, among others.

Bromophenol blue is a dye that changes color in response to changes in pH. At a pH of 3.0, it appears yellow, while at a pH of 4.6, it appears blue. This property makes it useful as a pH indicator in spectrophotometry, as the change in color can be easily measured using a spectrophotometer.

To use bromophenol blue in spectrophotometry, a solution of the compound is prepared in a cuvette, which is a small, clear glass or plastic tube used to hold samples for analysis. The cuvette is placed in the spectrophotometer, which measures the amount of light absorbed by the solution at a specific wavelength. The absorbance of the solution is then plotted against the concentration of the substance being measured, which allows for the determination of the concentration of the substance in the sample.

One of the advantages of using bromophenol blue in spectrophotometry is that it is a highly sensitive indicator, meaning that it can detect very small changes in pH. This makes it useful for applications where precise measurements are required, such as in biochemistry, where pH plays a critical role in many biological processes.

In addition to its use as a pH indicator, bromophenol blue is also used as a dye in the production of textiles, plastics, and paper, as well as in the manufacture of cosmetics and other personal care products. It is also used in the laboratory as a tracking dye in electrophoresis, a technique used to separate molecules based on their size and charge.

Overall, bromophenol blue is a valuable compound that has many important applications in a variety of fields, including spectrophotometry, where it is used as a pH indicator. Its sensitivity, ease of use, and versatility make it a valuable tool for researchers and analysts working in a wide range of fields.

Spectrophotometric Determination of the pKA of Bromophenol Blue (1).docx

bromophenol blue spectrophotometry

Part 4: Determination of effect of concentration on absorbance of methyl orange solutions Mixtures of distilled and methyl orange are prepared according Table 1. . A graph of absorption spectrum was plotted and the wavelength with maximum absorbance reading was determined from the graph. The first method requires knowledge of equilibrium concentrations of the reagents whereas the second method does not require that knowledge because it can be seen from the. The blue line indicates absorbance at 435.

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Using Spectrophotometer to measure the concentration of compounds

bromophenol blue spectrophotometry

Somehow, not all point is inside the straight line. Part 2: The effect of concentration of bromophenol blue on absorbance Table 1. The random error that might occur was that traces of chemical were present in the cuvette thus contaminating the distilled water which in turn giving the distilled water an absorbance reading. Molar absorbtivity coefficient of bromophenol blue in Amax of methyl orange is 0. In compounds that are ruled by basic and acidic groups the pK A controls numbers of aspects in metabolism and other important vital chemical processes in human body.

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Bromophenol Blue Using Spectrophotometry Lab Report

bromophenol blue spectrophotometry

First we added 1 mL of 0. This random error can be reduced by ensuring that the surface of the cuvette was wiped with paper towel before placing it into the spectrophotometer. The results is recorded in Table 1. The concentration of bromophenol blue in Tube C is 7. Copy to Clipboard Reference Copied to Clipboard.


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Wavelength Functions Of A Spectrophotometer

bromophenol blue spectrophotometry

In that equation, the x means concentration and the y absorbance. The absorbances of the two bromophenol blue of unknown concentrations were measured at the Amaxof bromophenol blue. Concentration of bromophenol blue in tube 1-6 was calculated using information in table 1. Part 4, the absorbance of light is directly proportional to the concentration of methyl orange as the standard concentration curve of absorbance against concentration of methyl orange shows a straight line passing through the origin. This random error can be reduced by ensuring that the surface of the cuvette was wiped with paper towel before placing it into the spectrophotometer. The first method used was interpolating the graph 2 while the second method used was using the formula of Beer-Lambert Law to calculate the concentrations of the two unknown solutions.

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Bromothymol Blue Spectrophotometry

bromophenol blue spectrophotometry

The absorbance of the bromophenol blue solutions in tubes 1-6 in Table 1. Furthermore , there might be fingerprint left on the wall of cuvette. This indicated that the absorbance of bromophenol blue is directly proportional to the concentration of bromophenol blue. This indicated that the absorbance of bromophenol blue is directly proportional to the concentration of bromophenol blue. Therefore, the Amax of bromophenol blue is in wavelength of 590nm.


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Bromophenol blue

bromophenol blue spectrophotometry

The first method used was interpolating the graph 2 while the second method used was using the formula of Beer-Lambert Law to calculate the concentrations of the two unknown solutions. The absorbance is then decrease when the wavelength applied was 620nm until 680 nm. Graph of standard concentration curve of absorbance versus concentration of methyl orange is plotted. The reading with concentration 6 x 10-4 M at wavelength 440 nm was discarded as it is too off from the trend line that it is not following the trend at all. As calculated before, the molar absorbtivity coefficient of methyl orange in Amax of bromophenol blue 590nm is 0.

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Spectrophotometry

bromophenol blue spectrophotometry

Distilled water should shows zero absorbance reading. By solving simultaneous equation, the concentration of bromophenol blue in the mixture C was 7. A graph of absorbance of light against concentrations of bromophenol blue is plotted. Figure 3- A graph showing the relation between concentration of bromophenol blue in M and their absorbance for the basic form of the molecule, showing the molar absorptivity constant of the molecule at wavelengths 440 nm and 590 …show more content… The molar absorption constants were found to be: 358 for the. You will be using micropipettes to combine various volumes of water and solutions of a blue dye, bromophenol blue BPB.

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Using Spectrophotometer to measure the concentration of compounds

bromophenol blue spectrophotometry

Conclusion Part 1, the Amax of bromophenol blue is at the wavelength of 590nm. The random error that might occur was that traces of chemical were present in the cuvette thus contaminating the distilled water which in turn giving the distilled water an absorbance reading. Share this: Facebook Facebook logo Twitter Twitter logo Reddit Reddit logo LinkedIn LinkedIn logo WhatsApp WhatsApp logo The spectrophotometer can measure the intensity of light absorbed indirectly by the solutes in solution because each solution has its own characteristic absorption chattels. However, there is some point that plotted far from the straight line. Tube C Amax of bromophenol blue 0.

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[PDF] Use of bromophenol blue in the spectrophotometric and turbidimetric determination of mebrophenhydramine in tablets

bromophenol blue spectrophotometry

By graphing pH vs. It works by analyzing the pattern of wavelengths absorbed by the sample. Mean while, using the formula of Beer — Lambert Law, the concentration of bromophenol blue in Tube A is 3. A graph of absorbance against concentration of methyl orange is plotted. These measurements from the diluted HCl and NaOH solutions provide us with an absorbance that is almost 100% HIn and another that is almost 100% In-, respectively. Part 2, the absorbance of light is directly proportional to the concentration of bromophenol blue as the standard concentration curve of absorbance versus concentration of bromophenol shows a straight line passing through the origin.

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