Photosynthesis lab elodea and bromothymol blue answers. Elodea Photosynthesis Lab 2022-11-05

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Photosynthesis is the process by which plants, algae, and some bacteria convert sunlight into energy. This process involves the conversion of carbon dioxide and water into glucose, a sugar, and oxygen. During photosynthesis, chlorophyll, a pigment found in the chloroplasts of plant cells, absorbs light energy and converts it into chemical energy.

One way to study photosynthesis is through the use of the aquatic plant Elodea and the pH indicator Bromothymol Blue (BTB). BTB is a weak acid that changes color in response to changes in the pH of a solution. When the pH is below 7, BTB is yellow. When the pH is above 7, BTB is blue.

In a photosynthesis lab, Elodea plants are placed in test tubes or other containers filled with BTB solution. The plants are then exposed to various conditions, such as different levels of light intensity or the presence of carbon dioxide, to observe the effect on photosynthesis.

For example, in one experiment, the Elodea plants may be placed in a dark room to simulate low light conditions. The BTB solution in the test tubes will initially be yellow due to the presence of carbon dioxide produced by the plants during respiration. However, as the plants undergo photosynthesis, they will consume the carbon dioxide and produce oxygen, causing the pH of the BTB solution to increase. This will result in a change in color from yellow to blue, indicating that photosynthesis is occurring.

In another experiment, the Elodea plants may be placed in a room with high levels of light intensity. Under these conditions, the plants will undergo photosynthesis at a faster rate, leading to a more rapid consumption of carbon dioxide and production of oxygen. This will result in a more pronounced change in the color of the BTB solution from yellow to blue.

By performing these experiments and observing the changes in the color of the BTB solution, students can learn about the factors that affect photosynthesis and the role of chlorophyll in this process. In addition, they can develop an understanding of the importance of photosynthesis in the lives of plants and the role it plays in the global carbon cycle.

Regular Photosynthesis Lab

photosynthesis lab elodea and bromothymol blue answers

On the other hand, one might expect respiring plants to release small levels of CO 2 into the water, causing the pH to decline slightly. These reactions absolutely require light, and are thus described as the "light-dependent reactions". Warning is hereby given that not all Project Ideas are appropriate for all individuals or in all circumstances. Create a bar graph to illustrate your results. In the dark, only cellular respiration occurs. Chlorophyll is a pigment in the chloroplast of the plant cell that absorbs energy from light and uses it to create compounds needed in photosynthesis. Write a hypothesis to explain which color light will be best for photosynthesis and why.

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elodea_lab_intro (1).docx

photosynthesis lab elodea and bromothymol blue answers

Thus, plants, including elodea, like to "do it with the lights on". Make sure that each piece looks healthy and has plenty of green leaves. As light intensity increases distance between lamp and plant decreases the volume of oxygen or the rate of bubble production increases. However, at sufficiently high levels of light intensity, the rate of oxygen evolution remains constant. Reading and following the safety precautions of all materials used in a project is the sole responsibility of each individual.


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Photosynthesis Lab

photosynthesis lab elodea and bromothymol blue answers

Cover each test tube or glass with plastic wrap. Carbon dioxide combines with water to form carbonic acid H 2 CO 3 which dissociates into hydrogen ions H + and bicarbonate ions HCO 3 -. Chlorophyll absorbs the energy of sunlight, and that energy is converted to chemical energy ATP and reducing power NADPH. The pH drops due to the presence of hydrogen ions. Record the initial color of the bromothymol blue solution.

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Elodea Photosynthesis Lab

photosynthesis lab elodea and bromothymol blue answers

In this experiment, the student will place aquatic plants under different colors of light in a solution of bromothymol blue. Set up the lamps at least several feet apart and away from windows. Note: the amount of CO 2 uptake in plants that are photosynthesizing far exceeds the amount of CO 2 released through respiration. Certain wavelengths of light are used by plants more than others. This will be assessed indirectly by measuring the amount of CO 2 given off or taken up by the plant. Plants use carbon dioxide and produce oxygen gas during photosynthesis. Write the name of the colored film to be tested on each cup you can also use masking tape to label cups.

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photosynthesis lab elodea and bromothymol blue answers

Just be sure to use distilled water and dilute the entire supply only slightly. By accessing the Science Fair Project Ideas, you waive and renounce any claims against Education. They produce carbon dioxide during cellular respiration. Carbon dioxide bubbled into water forms carbonic acid. Leave one lamp without a colored film as a control. In addition, your access to Education.

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photosynthesis lab elodea and bromothymol blue answers

Implementation of any Science Project Idea should be undertaken only in appropriate settings and with appropriate parental or other supervision. Bromothymol blue is an acid-base indicator that turns yellow in acidic solutions and blue in alkaline solutions. Record the final color of the bromothymol blue solution for each color of light, as well as both controls. This must occur in order for a plant to accumulate biomass, and be productive. The equation for this process, photosynthesis , is depicted below.

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photosynthesis lab elodea and bromothymol blue answers

Thus, the more photosynthetic activity, the less acidic and more blue the bromothymol blue solution becomes as the plant uses carbon dioxide during photosynthesis. Place a piece of elodea in each test tube or glass. This is not completely true, as the biochemical pathways and energy forms differ, however many of the enzymatic steps used in both processes are the same. Plants perform photosynthesis to create sugars and other organic compounds that they need to survive. Carbon dioxide from the atmosphere is then systematically reduced to carbohydrate using the ATP and NADPH generated in the light-dependent reactions. The less photosynthetic activity, the more acidic and yellow the bromothymol blue solution becomes as the plant produces carbon dioxide during cellular respiration.


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photosynthesis lab elodea and bromothymol blue answers

Be sure that the film is heat resistant to avoid fire hazard. The plants should all be 12 inches 30 cm away from their lamp. In today's lab, we will study photosynthesis in elodea in the light, and will also study background respiration by observing elodea in the dark. For further information, consult your state's handbook of Science Safety. Compare your results to your hypothesis.

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photosynthesis lab elodea and bromothymol blue answers

In the process, H 2 O is split and O 2 is released as a by-product. Allow the plants to remain under the lamps until the uncovered control has turned dark blue about 24-48 hours. This indicates that the rate of photosynthesis increases with light intensity. For one of the control plants, cover the test tube or glass completely with aluminum foil to block out any light. Place the remaining plants each under a colored lamp. Photosynthesis and Cellular Respiration in Elodea Elodea is an aquatic plant that uses the energy from sunlight to synthesize carbohydrate from carbon dioxide.

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photosynthesis lab elodea and bromothymol blue answers

CO 2 + H 2 O H 2 CO 3 H + + HCO 3 - During photosynthesis, plants would take up CO 2 from the water; we would therefore expect to see an increase in the pH of the water. Although these reactions are called the "light-independent reactions", plants do not perform these reactions in the dark; they perform them in the light, as long as sufficient ATP and NADPH and CO 2 are available. When plants are exposed to light, photosynthesis and cellular respiration both occur. Be sure to completely seal the vessel to keep gas from entering or leaving. C 6 H 12 O 6 + 6O 2 6CO 2 + 6H 2 O + Chemical Energy ATP Notice that these two equations appear to be opposites. Secure the film with tape.

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