Antisense rna technology. Antisense RNA 2022-11-07

Antisense rna technology Rating: 8,6/10 143 reviews

Antisense RNA technology is a revolutionary approach to gene regulation that involves the use of small RNA molecules to inhibit the expression of specific genes. This technology has the potential to revolutionize the way we treat diseases and conditions caused by abnormal gene expression, and has already been used in a number of clinical trials for the treatment of various diseases.

The basic principle behind antisense RNA technology is simple. When a gene is transcribed into RNA, the RNA molecule is then translated into a protein. Antisense RNA technology involves the use of small RNA molecules that are complementary to the RNA transcribed from a specific gene. When these small RNA molecules bind to their complementary RNA target, they inhibit the translation of the gene into protein.

There are several different types of small RNA molecules that can be used in antisense RNA technology, including microRNAs, short interfering RNAs, and small activating RNAs. Each of these types of small RNA molecules has its own specific mechanisms of action and can be used to regulate gene expression in different ways.

One of the main advantages of antisense RNA technology is that it can be highly specific, targeting only the RNA transcribed from a specific gene. This means that it can be used to inhibit the expression of a single gene without affecting the expression of other genes. This specificity makes it an attractive option for the treatment of diseases that are caused by abnormal gene expression, such as cancer or genetic disorders.

Antisense RNA technology has already been used in a number of clinical trials for the treatment of various diseases. For example, it has been used to treat cancer by inhibiting the expression of specific genes that are involved in the growth and proliferation of cancer cells. It has also been used to treat genetic disorders, such as sickle cell anemia, by inhibiting the expression of the abnormal gene responsible for the disorder.

Despite the promising results of these clinical trials, there are still many challenges to overcome in the development of antisense RNA technology. For example, one of the main challenges is delivering the small RNA molecules to the appropriate target cells in the body. Another challenge is ensuring that the small RNA molecules remain stable in the body and do not degrade before they can be effective.

Overall, antisense RNA technology represents a promising approach to gene regulation and has the potential to revolutionize the way we treat diseases caused by abnormal gene expression. While there are still many challenges to overcome, the potential benefits of this technology make it an exciting area of research with a bright future ahead.

Antisense technology: an overview and prospectus

antisense rna technology

Antisense drug discovery and development technology considered in a pharmacological context. Cellular uptake mediated by epidermal growth factor receptor facilitates the intracellular activity of phosphorothioate-modified antisense oligonucleotides. As described in the cis-acting asRNAs, the mRNA-asRNA pairing can result in blockage of ribosome entry and RNase H dependent degradation. Improvements in the design and chemistry of antisense compounds have enabled this technology to become a routine tool in basic research, genomics, target validation, and drug discovery. Retrieved 7 March 2021.

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Antisense RNA

antisense rna technology

For more than 100 years, most traditional medicines like small molecule inhibitors or, more recently, antibody-based therapies have worked to target these proteins once they are produced and doing damage in the body. Typically, ASOs are 15—22 nucleotides in length and are designed to bind complementary RNA targets, ultimately facilitating their degradation via the RNase H pathway. There is a strain of rice LGC-1 that produces abnormally low levels of proteins called glutelins. Recent years the idea of targeting asRNAs to increase gene expression in a locus specific manner has been drawing much attention. These small single-stranded transcripts are generated by the cleavage of larger precursors using the C. Somatic tissues of plants, e. The medicinal chemistry of therapeutic oligonucleotides.

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Ionis' antisense technology to be featured during virtual RNA at the Bench and Bedside II Conference

antisense rna technology

ASOs in development are showing substantial improvements in potency and performance based on advances in medicinal chemistry, understanding of molecular mechanisms and targeted delivery. The current state and future directions of RNAi-based therapeutics. Thus repression of gene expression by miRNAs appears to be a mechanism to ensure regulated and coordinated gene expression as cells differentiate along particular paths. Sometimes, however, gene repression can be achieved by prematurely terminating or slowing down transcription process. Because the cell can continuously synthesize shRNA, the interference is long-lasting. This may occur because the ribosome cannot gain access to the nucleotides in the mRNA or because the duplex RNA is quickly degraded by ribonucleases in the cell. Cellular and Molecular Life Sciences.

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Antisense RNA Technology

antisense rna technology

Such amplification of an initial trigger signal suggests a catalytic effect. . This paper shows that chemically modified single-stranded oligonucleotides can act as siRNAs and activate the RNA-induced silencing complex RISC pathway in vitro and in vivo. The IDT We recommend at least two qPCR assays designed a distance apart from each other on the transcript to help control for artifacts. After 24 hours, RNA levels were measured by RT-qPCR.

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[PDF] Application of antisense RNA technology for crop improvement; A review

antisense rna technology

Multivalent N-acetylgalactosamine-conjugated siRNA localizes in hepatocytes and elicits robust RNAi-mediated gene silencing. Amplification of RNAi In C. Retrieved 12 December 2019. The use of 5-methyl dC in CpG motifs can also reduce the chance of adverse immune response to Toll-like receptor 9 TLR9 in vivo. The region responsible for this repression function was found to be a 300 base-pair locus upstream of the ompC promoter.

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Antisense and RNAi Therapeutics Market

antisense rna technology

Such a universal cell response must have an important function. Therapeutic antisense oligonucleotides are coming of age. In fact, the suppressive effect of antisense RNA probably also depends on its ability to form dsRNA using the corresponding mRNA as a template. With the aid of a protein, it binds to a complementary sense sequence on a molecule of mRNA. Probably each miRNA can bind to as many as 200 different mRNA targets while each mRNA has binding sites for multiple miRNAs.


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Antisense therapy

antisense rna technology

This is one of a series of papers describing the designs of PS ASOs that can cause target reduction via non-RNase-H1 mechanisms. Advances in antisense oligonucleotide development for target identification, validation, and as novel therapeutics. Retrieved 18 December 2019. Antisense therapies can also treat diseases caused by too little protein by increasing the production of the protein, thereby restoring the protein to normal levels. National Library of Medicine. An example was E. Substitution of 5-methyl dC for dC will slightly increase the T m of the antisense oligo.

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What is Antisense Technology?

antisense rna technology

In plants, the gene silencing spreads to adjacent cells through plasmodesmata and even to other parts of the plant through the phloem. ASOs are designed to the pre-mRNA sequence, and therefore, are complementary to the exon and intron junction. The ability of dsRNA to suppress the expression of a gene corresponding to its own sequence is called RNA interference RNAi. Drugs 80, 1027—1031 2020. Transgenic tomatoes have been constructed that carry in their genome an artificial gene DNA that is transcribed into an antisense RNA complementary to the mRNA for an enzyme involved in ethylene production.

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11.10: Antisense RNA

antisense rna technology

Antisense therapies are designed to seek out, bind to and destroy a mRNA in a highly specific manner, so that the amount of disease-causing protein is dramatically decreased. RNA interference RNAi In testing the effects of antisense RNA, one should use sense RNA of the same coding region as a control. The ease with which miRNAs can be introduced into cells and their widespread effects on gene expression have given rise to hopes that they might be useful in controlling genetic disorders, e. Retrieved 12 August 2020. MicroRNAs miRNAs In C. Summary In addition to protein transcription factors, eukaryotes use small RNA molecules to regulate gene expression — almost always by repressing it — so the phenomenon is called RNA silencing.

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